In recent years, mesenchymal stem cells mscs have emerged as a promising therapy for periodontal regeneration in animal studies 4, 5 and in human studies 6. Promising treatment for periodontitis gum regeneration. When considering application to periodontal regeneration, stem cells derived from the tissues surrounding the teeth, i. Other stem cells, such as bone marrow stromal stem cells and adipose tissue stem cells. Mesenchymal stem cells and periodontal regeneration.
Their most common source is the bone marrow hematopoietic stem cells or bone marrow stromal cells mesenchymal stromal stem cells. The regeneration of lostdamaged support tissue in the periodontium, including. Periodontal regeneration in swine after cell injection and. The purpose of the present study is to evaluate the effect of cell injection and cell sheet transplantation on periodontal regeneration in a swine model. Periodontal regeneration using periodontal ligament stem. Enhancement of periodontal tissue regeneration by transplant of bone marrow mesenchymal stem cells. Periodontal ligament stem cells for periodontal regeneration. Despite unmet clinical needs for reconstruction of tissues lost in congenital anomalies, infections, trauma, or tumor resection, how orofacial stem progenitor cells contribute to tissue development, pathogenesis, and regeneration. The identification and manipulation of stem cells has greatly advanced regenerative medicine and has contributed to development of tissue engineeringbased clinical therapies 19. Stem cells in gum regeneration and lanap preserve your. Stem cells and periodontal regeneration wiley online library. Advances in stem cell biology and regenerative medicine have presented opportunities for tissue engineering and genebased approaches in.
Mesenchymal stem cell exosomes enhance periodontal. The scope of stem cells in periodontal regeneration. Gene therapy, stem cells, and rna interference periodontal disease is a major public health issue and the development of effective therapies to treat the disease and regenerate periodontal. This setting creates a healing coagulum containing the stem cells from patients own bone marrow. Among the various stem cell types used for cytotherapy and tissue engineering, mscs are currently proposed as an attractive cell source for bone and tooth regeneration due to their potential. Periodontal disease is characterized by the destruction of tooth supporting tissues. Chronic periodontitis is an inflammatory disease, resulting in. Periodontal ligament stem cells pdlscs, which reside in the perivascular space of the periodontium, possess characteristics of mesenchymal stem cells and are a promising tool for periodontal regeneration. Mscs were initially discovered in bone marrow, and bone marrow mesenchymal stem cells bmmscs were found to promote periodontal regeneration when. Colgate australian clinical dental research centre, the university of adelaide, south. Utilizing human gmscs as a promising source for periodontal tissue regeneration.
Scaffold technology and biomaterials for stem cell based tissue regeneration in the oral and maxillofacial region. Since then, other stem cells have been found to possess the ability to form multiple periodontal tissues. We developed a novel cell transplant method for periodontal regeneration using periodontal ligament stem cell. The identification of stem cells from human pdl tissues, termed pdl stem cells pdlscs, in 2004, led to a new era of research on periodontal regeneration 14. Review article critical issues in periodontal regeneration. To develop a novel regenerative and adhesive hydrogel encapsulating patients gingival stem cell which can potentially be used as an adhesive dental hydrogel for periodontal tissue regeneration. Recently, great progress has been made in pdlsc transplantation. Curr oral health rep induced pluripotent stem cells and periodontal regeneration mi du 0 1 xuejing duan 0 1 pishan yang 0 1 0 department of stomatology, shandong provincial hospital affiliated to shandong university, no. Periodontal regeneration an overview sciencedirect topics. These last can be potential candidates for periodontal regeneration 15.
It also covers various experimental strategies that could be used to direct lineagespeci. The use of stem cells for periodontal regeneration is a hot field in. Stem cells from the periodontal ligament pdlscs are promising for periodontal regeneration because they can undergo guided differentiation under specialized conditions. Numerous types of stem cells have been isolated from dental tissue, such as dental pulp stem cells dpscs, stem cells isolated from human pulp of exfoliated deciduous teeth shed, periodontal ligament stem cells pdlscs, stem cells from apical papilla scaps, and dental follicle cells dfcs. Those stem cells are then added to the decontaminated pocket and seal the gum tissues against the tooth root, promoting tissue regeneration. The destruction and loss of periodontal fixation is a worldwide medical concern and is one of the leading causes of tooth loss, representing a critical challenge for the clinical regenerative. In the last step, the uses a specific setting on the laser.
Stem cells and periodontal regeneration request pdf. Postnatal orofacial tissues harbor rare cells that exhibit stem cell properties. Abstractperiodontal regeneration is considered to be biologically possible but. Guided tissue regeneration one of the crucial cellular events is recruitment of cells as the cell type selection determines whether healing occurs by repair or regeneration. Induced pluripotent stem cells and periodontal regeneration. Pdf cell and gene based therapeutics for periodontal. Pdlscs are a kind of somatic stem cells that show potential to differentiate into multiple cell. They can differentiate into endothelial, perivascular, neural, bone or muscle cells. Chronic periodontitis is a disease of the periodontium characterized by irreversible damage in connective tissue attachment and supporting alveolar bone. Progenitor and stem cells are of particular interest in periodontal wound healing and regeneration as they are most likely the parental cells of synthetic cells e. The ultimate goal of periodontal therapy is the regeneration of these lost structures. The goal of periodontal treatment is to control the infections and reconstruct the structure and function of periodontal tissues including cementum, periodontal ligament pdl fibers, and bone. More recently, several small human clinical trials have used periodontal ligament stem cells and dental pulp stem cells.
Request pdf stem cells and periodontal regeneration periodontitis is an inflammatory disease which manifests clinically as loss of supporting periodontal tissues including periodontal ligament. School of dentistry, the university of adelaide, south australia. This article is from balkan journal of medical genetics. These structures are the target of most common dental diseasesdental caries and periodontal diseases. Faming chen, songtao shi, in principles of tissue engineering fourth edition, 2014. Weir 3, ke ren 4, abraham schneider 5,6, ping wang 3, thomas w. The pdl is a fibrous connective tissue that contains specialized cells. Periodontal regeneration by allogeneic transplantation of adipose. Terminology glossary of periodontal terms a graft is any tissue. Stem cells used so far in periodontal regeneration have been identified, and their characteristics that aid in periodontal regeneration, and various methods used to incorporate them examined in detail. Gingival mesenchymal stem cells as a novel treatment.
These structures are the target of most common dental diseasesdental caries and periodontal. Stem cells, tissue engineering and periodontal regeneration. To date, different human dental stem cell progenitors have been identified and isolated bone marrow stromal cells bmmsc, human pulp tissue. One of the critical requirements for a tissue engineering approach is the delivery of ex vivo expanded progenitor populations or the mobilization of endogenous. Stem cells are the latest entrant in periodontal regeneration. The use of mesenchymal stem cell msc therapy offers the potential to develop a completely novel and improved method of periodontal regeneration compared to existing methods. Periodontal regeneration by allogeneic transplantation of. The dental complex is a multistructure organ composed of highly mineralized tissues i. We consider and describe the main stem cell populations that have been utilized with regard to periodontal regeneration, including bone marrowderived. Periodontal ligament stem cells pdlscs possess the capacity of selfrenewal and multilineage differentiation and are likely to recover lost alveolar bone. In the present study, human dental pulp stem cells hdpscs were transplanted into a swine model for periodontal regeneration. During tooth development, the periodontal stem cells. Dental stem cell and dental tissue regeneration springerlink. A novel approach for periodontal tissue regeneration is to employ different populations of dental stem cells in order to recapitulate key events during periodontal tissue development.
Application of stem cells in the oral and maxillofacial. The discovery of periodontal ligament stem cells pdlscs highlighted the possibility for development of tissue engineering technologybased therapeutics for disrupted periodontium. The field of regenerative medicine is discovering how to use stem cells to stimulate the body to generate new tissue to replace areas affected and damaged by disease. Osteoblasticcementoblastic and neural differentiation of. Regeneration of periodontal tissues using ex vivo expanded cells has been introduced and studied, although appropriate methodology has not yet been established. Characterization and culture of stem cells derived from the oral and maxillofacial region e. Mesenchymal stem cells, periodontal ligament stem cells, periodontal regeneration. Periodontitis is a prevalent infectious disease worldwide, causing the damage of periodontal support tissues, which can eventually lead to tooth loss. This kind of treatment paradigm is important because commercially available stem cells that represent a cell based therapy specifically developed to treat periodontal tissue regeneration will. At the cellular level, pr is a complex process that requires coordination between the proliferation, differentiation and development of various cell types. Pdf stem cells, tissue engineering and periodontal.